This is a pretty important aspect of microbiology: necessary to avoid contamination, or cross contamination; good old observation of strict aseptic technique.
Now you don’t necessarily have to observe strict protocols or even adhere to ‘strict’ aseptic technique, but if you are advised to work around a bunsen, flame tweezers before use, flame lids and keep tip boxes closed between use, then you’ll probably avoid most contamination risks. Well if you don’t listen, this is what you end up with..
I’m pretty laid back about working habits and don’t get contamination, but I’m also impressed that it’s even possible to …
And so the practical SSC for undergraduate medics started and finished this week. Such a lovely group of students – very knowledgable, inquisitive and what I think was genuinely interested in oral microbiology!
The adherence assay is not a particularly challenging experiment to do, but for those that have no lab experience, it can still be quite exciting. The intro lecture was pretty good too – covered quite a lot of background, and hopefully passed on some knowledge and expertise of my own. Now they have a presentation of their own to think about and prepare for..
They say s PhD journey is 10% intelligence and 90% persistence and perseverance, and to be honest, I’m very lucky to have the project and supervision that I do. However, I would liked to have had this the other way around – not being able to get things to work in the first year, only to then generate a ton of data in the following years. It’s also times like these that the ‘imposter syndrome’ begins to rear its ugly head; why am I here when I can’t get s simple experiment to …
So the plan for the past few days was to keep my cells growing (enough to use them soon if they grow quick enough!), do a bit of monocyte work and set up and maintain biofilms.. and it has once again proved to be a somewhat challenging but rewarding week at that.
Cells have established themselves very well indeed, and I’ve managed to grow enough to have two sets of freezer stocks, and two flasks actively growing, and the keratinocytes are doing pretty well too 🙂 (albeit significantly slower growing than the fibs!). Monocyte work didn’t really go to plan..as …
Whats happened since?
– I attended OMIG at Gregynog, which was my first conference. I presented a poster and won the poster prize! #Win
– I completed the MEDIC SSC project with grate success
– I attended the MITReG Conference in Cardiff and won second prize for my poster
– I passed my first year progress monitoring with no problems 😀
– A paper on which I was second author was published int he journal Biofouling. It encompasses my first years work and having spent an amazing year working with Yuri …