Lab work

Hard at work, or hardly working? 

Hard at work, or hardly working? 

That has always made me chuckle. And for those who are concerned, hard at work, all the time ;). But the past two weeks have been particularly super busy! Mainly in the lab. That’s a good thing I guess, in some ways at least. It means you can’t get distracted, but by working in the lab you are by definition distracted from writing.

This week, Josh and I had some tissue models come in for infections, so we grew biofilms as we do for a few days, and then infected the tissue models. All 72 of them!!

Luckily we can …

I can see clearly now

I can see clearly now

The leading statement ‘I can see clearly now’ has dual purpose. Firstly, I have new glasses 😀 20161019_165146

I haven’t had glasses since I was really young – probably 20 years ago now (man that makes me feel old..). I should have continued wearing them through my childhood and maybe I wouldn’t have needed them now!! Oh well. Ruben has glasses, and if he can pull them off as well as he does, I’ll give them a go. So I went to have my eyes tested, and sure enough I still need them (believe it or not). Heres the technical part …

Summertime!

Summertime!

And so the summer is here, kids are off school for summer, people going on summer holidays, the sun is out (or was for a few days at least), and people are generally in a happier mood :). And for those budding microbiologists of you out there, you know the time where you can grow your bugs on the bench instead of the incubator 😉 Come on, you’ve all done it….

Win win, right?

Unfortunately, science doesn’t know what summer is, and doesn’t know what a holiday is. But it’s not all bad! Currently I’m at all systems go! My …

It is nearly time, but there is never enough time!

It is nearly time, but there is never enough time!

This week has been a hectic one to say the least, and we’re only half way through!!

The week started with poster printing for the Biofilms7 conference this weekend (25-28 June) in Porto, Portugal. All sorted and poster collected, I then had another run of host cell responses to microorganisms underway. Seeded the cells ready to go..we’ll see how that goes in the next couple of days..

Tuesday came and went really quickly, Get into work and then straight on with challenging my cells with various conditions for the responses expt. Had a course booked, but with everything that was …

No more hand-me-downs

No more hand-me-downs

NEW PIPETTES! Most often is the case that researchers join an already established group, where they inherit equipment, consumables, reagents etc, and included in this are pipettes. They may be old, uncalibrated, unchecked, but still used. In a scientists day to day life, here are few things quite as exciting as getting new pipettes! Not very often do you get an opportunity to browse, test and evaluate pipettes and then get a new set! Of course they belong to the group, and I won’t be the only one using them but they’re amazing and I’m so chuffed 🙂 I can’t …

If it looks too good to be true

If it looks too good to be true

Then you’ve probably done something wrong! Like picking up a pack of chicken breasts at £1.15 thinking you have a real bargain, when they are actually £1.15 for each breast…so £4.60 in total!! But you buy two packs because you’ve no idea, and get home to be told what an idiot you really are!! Or adding too high a concentration of standard! In this case anyway…
Looking at host cell responses for cytokine production of cells after microbial challenge, the ELISA to quantify this cytokine worked all too well. The concentration of the standard was way too high for the …

Aseptic technique..

This is a pretty important aspect of microbiology: necessary to avoid contamination, or cross contamination; good old observation of strict aseptic technique.

Now you don’t necessarily have to observe strict protocols or even adhere to ‘strict’ aseptic technique, but if you are advised to work around a bunsen, flame tweezers before use, flame lids and keep tip boxes closed between use, then you’ll probably avoid most contamination risks. Well if you don’t listen, this is what you end up with.. 

 I’m pretty laid back about working habits and don’t get contamination, but I’m also impressed that it’s even possible to …

When the PhD gives you lemons..

When the PhD gives you lemons..

..make sure you get at least n=3. 

They say s PhD journey is 10% intelligence and 90% persistence and perseverance, and to be honest, I’m very lucky to have the project and supervision that I do. However, I would liked to have had this the other way around – not being able to get things to work in the first year, only to then generate a ton of data in the following years. It’s also times like these that the ‘imposter syndrome’ begins to rear its ugly head; why am I here when I can’t get s simple experiment to …

‘Flow’ing like a river

‘Flow’ing like a river

So the plan for the past few days was to keep my cells growing (enough to use them soon if they grow quick enough!), do a bit of monocyte work and set up and maintain biofilms.. and it has once again proved to be a somewhat challenging but rewarding week at that.

Cells have established themselves very well indeed, and I’ve managed to grow enough to have two sets of freezer stocks, and two flasks actively growing, and the keratinocytes are doing pretty well too 🙂 (albeit significantly slower growing than the fibs!). Monocyte work didn’t really go to plan..as …

This week in review

This week in review

What a week!

It didn’t feel particularly busy, because working with cells means a lot of waiting for them to…well, grow. It’s all the little bits that seem to add up and make what seems like it should be an average week, into a very not average week.

Did some flow work at the start of the week which showed the new antibody I bought in worked :D. I also had another clinical sample – a stomatitis one this time too, so excited to see what comes back off the plates for isolated bugs..and need to sort the DNA extraction …