Lab work

Highs and lows and highs

Highs and lows and highs

This week was filled with anticipation; ELISA kits due to arrive, antibodies due to arrive, tissue models ready to be used for infections.. However, it quickly became apparent that this week was going to be a tough one!

After chaining the medium of the tissue models (all 36 of them..) on Monday, all was well. Until Wednesday. During the medium change on Wednesday, I noticed the medium was cloudy, and upon a quick look under the microscope I found the wells packed with what look like fungal spores, or yeast cells (Candida?)! Such a shame as these had been growing …

Back to it!

So..been a while since my last post!

Whats happened since?
2014:
– I attended OMIG at Gregynog, which was my first conference. I presented a poster and won the poster prize! #Win
– I completed the MEDIC SSC project with grate success
– I attended the MITReG Conference in Cardiff and won second prize for my poster
– I passed my first year progress monitoring with no problems 😀

2015:
– A paper on which I was second author was published int he journal Biofouling. It encompasses my first years work and having spent an amazing year working with Yuri …

Teaching and Learning, OMIG 2014

I really dislike PCR. There. I said it.

OK, maybe I don’t really dislike PCR, but I don’t like when experiments don’t work. I find it hard to acknowledge the fact that doing a PhD is 10% intelligence and 90% persistence..life is very different in industry; if it doesn’t work, you leave it, or try one other thing to make it work.

Academic research is very different.. it brings to mind the phrase “if at first you don’t succeed, try, try and try again”. However, I had made a monumentally ridiculous transcription error (see what I did there!). Having had …

Monday blues through to TGIF!

Monday morning came too quickly this week. Sat (what I hope to be) the last exam for he foreseeable future; Biomedical methods research techniques, and honestly..it was hard work! A lot about protein work, cell culture, bioinformatics but very little microbiology (one page of twelve total!) so not a particularly equal spread of questions over the course! Such a shame..but fingers crossed now until the results.

With the dreaded exam over, the rest of the week should really have been easy going – just get on with in vitro biofilm growth, confocal analysis on Wednesday, start again on Thurs and …

Catching up!

Wow, over three months since my last post..sorry about that!

So what have I been up to in the past three months?
– I’ve gotten stuck in with lab work; making acrylic pieces, doing adherence assays and growing biofilms on them (and looking at them using fluorescence confocal microscopy to determine depth and arrangement!). Also have done DNA extraction, run PCRs and purified samples (ready for sequencing) of my stock cultures.. all good fun but very important work for when my clinical samples start coming in!..
– Talking of clinical samples, IRAS (ethics) forms have been submitted and we are …